Web5. mar 2024 · 版权. 生物信息学习 专栏收录该内容. 6 篇文章 0 订阅. 订阅专栏. 原文章地址: http://ccb.jhu.edu/software/tophat/manual.shtml. 用法. tophat [options]* … Web9. nov 2024 · 在转录组定量分析时,如果采用的是alignment-based转录组定量策略,那么一般会使用的是HISAT2、STAR或者TopHat等比对软件。 接着则是对转录组进行定量,如果是基于基因水平的定量,我之前一般是采用HTSeq-count工具来获取每个基因上的count数。所谓count数,个人简单的理解为根据不同比对情况,将reads ...
tophat 原理_使用TopHat分析RNA-Seq结果 - CSDN博客
Web25. apr 2013 · TopHat2 combines the ability to identify novel splice sites with direct mapping to known transcripts, producing sensitive and accurate alignments, even for highly … WebIn the call to tophat2, the option -o specifies the output directory, -p specifies the number of threads to use (affect run times, vary depending on the resources available). The first argument is the name of the index. The second argument is a list of all FASTQ files. Other parameters can be specified to tophat2 : jeep of manhattan parts
tophat2+cufflinks进行转录组的比对分析 - 云+社区 - 腾讯云
Web1. máj 2009 · However, current software for aligning RNA-Seq data to a genome relies on known splice junctions and cannot identify novel ones. TopHat is an efficient read-mapping algorithm designed to align reads from an RNA-Seq experiment to a reference genome without relying on known splice sites. Results: WebTopHat是一个快速的将RNA-Seq数据进行快速剪接映射的程序,它使用超快的高通量短读比对程序,将RNA-Seq的信息比对到哺乳动物大小基因组上,然后分析映射结果来鉴别外显 … Web26. dec 2024 · tophat2+cufflinks转录组测序实例将为你介绍转录组测序也就是最近热门的RNAseq整个流程,有兴趣的小伙伴可以点个关注,一起讨论学习!. 人的基因组一共有两万多个基因,但这些基因并不是每时每刻都在表达,在不同时间不同组织中,基因的表达是不同 … jeep of memphis tn